FINAL MOLECULAR BIO
Mutagenesis - Dr Chau De Ming 1. How homolog study works for a newly discovered gene Compare unknown gene sequence with known gene sequence in other organism Use gene seq database Show similarities Reveal protein function of new gene Predict potential roles in cellular processes and pathways 2. How reporter gene works Track timing and location of a protein expression Green fluorescent protein Observation of GFP fluorescence when gene of interest is expressed Enable detection of gene and provide indication of transcriptional activity of the gene 3. Germ line mutation: First appear in gamete Passed from parents to offspring Found in every cell of offspring Somatic mutation: First appear in somatic cells Not inherited/passed Found in specific cells where mutation first originated 4. Point mutation: Affects single ntd base pair Silent Missense Nonsense ...