7. suruhanjaya, peranan, fungsi, sprm, spr, beza dng kementerian 006, 008 SPR: badan pengendali perjlnan pr samada di peringkat Dewan Rakyat (Parlimen) mahupun Dewan Undangan Negeri DUN dibentuk pd 4/9/1957 di bawah perutkan perkara 113,114 perlembagaan persekutuan peranan: mengendali sesebuah pr menjlnkan semakan persempadanan kawasan2 pr parlimen & negeri x kurng dr 8 tahun selepas tarikh siapnya urusan kajian & persempadan semula yg terdahulu jlnkan urusan pendaftaran serta menyemak & kemaskini senarai nama2 daftar pemilih bukan saja mendaftar pemilih yg baru tapi juga mengeluarkan nama pemilih yg telah tinggal dunia dng kerjasama JPN - hanya pemilih2 berdaftar berhak mengundi bertanggungjawab mengendalikan pr umum & rakyat kecil yakni melibatkan pemilihan ahli2 Dewan Rakyat & DUN termasuklah pr kecil jika ada kekosongan proses: pengeluaran writ - penamaan calon - kempen pr - pengundian - penjumlahan rasmi undian - pegumuman keputusan p...
Cell Counting = process of determining cells no 算细胞 importance, method, which method recommended importance of cell counting ensure accurate seeding densities 播种密度 monitors growth rates 成长 evaluates cytotoxicity or proliferation 细胞毒性与增殖 standardize experimental conditions methods manual counting hemacytometer slide requires a minimum of 1x10^6 cells/mL use trypan blue for viability test viable cells not stained cheap slow prone to error low sensitivity electronic counting original resistance-based counter based on the change in current generated when a cell passes through a narrow orifice 孔 image analysis software scan stained & unstained cells in a special counting chamber & view continuous monitoring of living cultures work by counting cells or determine residual available growth area rapid & low inherent 固有 error - more accurate needs single cell suspension flow cytometry count by light scat...
You are given a suspension of cells at a concentration of 5 × 10⁵ cells/ml. You are asked to plate the cells for single-cell cloning using a 24-well plate, where each well contains 500 μl of culture media and should ideally contain 1 cell. How many times should you dilute the original cell suspension to achieve this? 先要知道final是5×10⁵ initial就是说明一个well里有500ul=0.5ml=1 cells 那1ml就是2cells 所以initial是2cells/ul dilution factor套公式是final/initial=250k 怎么算dilution factor可以用either 250k=100*100*25 或 100*50*50 这时就是用总额1ml来算的话,应该是 10ul of 5×10⁵ + 990ul Media = 100x 10ul of 5×10³ + 990ul Media = 10000x 40ul of 5×10¹ + 960ul Media 其实也就是 5×10⁵ = 500k 5×10³=5k 5×10¹=50 500k÷5k÷50=2cells/ml volume是算DF得出来的 10ul+990ul=100x 1000/10 DF 40+960/40=25x 20+980/20=50x Primary culture b4 subculture acquisition, isolation, disaggregation, culture after seeding human use JKEUPM animal use IACUC mouse embryo: undifferentiated mesenchymal cells full term 19-21d organ form at 9d - cells w less tiss...
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