Practical Types of Cell Culture

 

  • Cell type
    • tissue of origin
    • morphology
    • specialized fx
  • 1 tissue > 1 cell type
  • specific cell type derived from differ tissue
    • smooth muscle cell from aorta, pulmonary, coronary
  • each cell type variety cell line
    • differ sp
    • finite→primary-senescence
    • continuous→established-tumor
  • Process
    • dissection & tissue isolation
    • tissue disaggregation→detach cells using enzymes, grow in culture vessels
    • enrichment→only cells wanted persist
    • culture→perform characterization to confirm cells
    • subculture max 3 flask
    • finite
    • continuous
  • ethics, identify donors, cooperation from clinicians
  • optimization of culture conditions, detailed records on cell handling=provenance
  • finite→after some subculture, increase til max cell no, drop=senescence
  • continuous=transformed→genotypic & phenotypic changes
  • Research obj
    • ds/condition
    • tissue & cell population or type
    • specific requirement
  • Factors
    • sp
    • finite vs continuous
    • normal vs transformed
    • history
    • growth rate, cell viability, morphology, mode of growth
    • culture requirement

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