Cranial Nerve

Cranial Nerve 



 

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CELL CULTURE

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You are given a suspension of cells at a concentration of 5 × 10⁵ cells/ml. You are asked to plate the cells for single-cell cloning using a 24-well plate, where each well contains 500 μl of culture media and should ideally contain 1 cell. How many times should you dilute the original cell suspension to achieve this? 先要知道final是5×10⁵ initial就是说明一个well里有500ul=0.5ml=1 cells 那1ml就是2cells 所以initial是2cells/ul dilution factor套公式是final/initial=250k 怎么算dilution factor可以用either 250k=100*100*25 或 100*50*50 这时就是用总额1ml来算的话，应该是 10ul of 5×10⁵ + 990ul Media = 100x 10ul of 5×10³ + 990ul Media = 10000x 40ul of 5×10¹ + 960ul Media  其实也就是  5×10⁵ = 500k 5×10³=5k 5×10¹=50 500k÷5k÷50=2cells/ml volume是算DF得出来的 10ul+990ul=100x 1000/10 DF 40+960/40=25x 20+980/20=50x Primary culture b4 subculture acquisition, isolation, disaggregation, culture after seeding  human use JKEUPM animal use IACUC mouse embryo:  undifferentiated mesenchymal cells full term 19-21d organ form at 9d - cells w less tiss...
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